Holed, bended, round shaped chromosomes may lead to poor chromatids labelling with one pair of gray seeds. Here with several pairs of seeds, a much better labelling is reached:The seeds used to get the result were choosen by hand:
The result depends on how the seeds were chosen. Four independent factors must be taken into account:
|Chromatids labelling after watershed|
- The pairs of seeds number:
- One pair yields poor results as observed previously.
- The orientation of the pair of seeds relative to the chromosome
- The axis of the pair of seeds, must be locally orthogonal to the axis of the chromosome.
- For a given pair of seeds, the distance between the two discs.
- The relative orientation of the pair of seeds
- The pair of seeds must have the same orientation
|seeds and edge of binary chromosomes in false colors.|
|Harlequin pattern (black holes are due to the absence of seeds to label the background)|