A model image of a metacentric chromosome was generated with ImageJ. The model is simplistic, it consists in X shaped binary image:
|16 bits binary image|
The initial location of the seeds, was in that first trial choosen by hand.
|Initial seeds location inside the chromosome|
|seeds after watershed yield labelled chromatids (light blue, dark blue)|
The result is nice because the initial location was purposefully chosen. What would happen with varying seeds location or orientation ?
|Seeds location shifted in the lower left chromatid|