Saturday, April 1, 2017

100 overlapping, full resolution, chromosomes isolated from 14 metaphases of human lymphocytes

Metaphasics chromosomes were hybrized with a PNA telomeric probe (CCCTAA-Cy3) and an additional oligo DNA probe (Cy5) for testing purposes.

100 overlapping chromosomes can be found in that dataset (jpp21). The dataset consists of 12 bits raw greyscaled images available on the DeepFISH repository. The images of overlapping chromosomes were isolated and converted into 8bits rgb images:

Sample of 100 overlapping chromosomes (full resolution)
The overlapping images are also available as combination of DAPI and Cy3 components:

Combination DAPI+Cy3 images (inversed greyscale)