MFISH image to RGB with image5D

Wade Schwartzkopf published a MFISH dataset  in 2000. Image5D (a plugin for imagej) can display five different fluorochromes simultaneously:

Left:raw png images. Top right: RGB image done with Image5D. Bottom right: "karyotype" image with false colors. 

Far red

Red

Spectrum gold

Spectrum green

Spectrum aqua

Dapi

After chromosome classification, a karyotype image can be produced. This is a grayscaled image associated to a  LUT, to be displayed with false colors:

Image5D can export a rgb image from the five channels image:

RGB image generated after background substraction of the five raw images