Thursday, May 19, 2011

MFISH image to RGB with image5D

Wade Schwartzkopf published a MFISH dataset  in 2000. Image5D (a plugin for imagej) can display five different fluorochromes simultaneously:
Left:raw png images. Top right: RGB image done with Image5D. Bottom right: "karyotype" image with false colors. 

Far red
Spectrum gold
Spectrum green
Spectrum aqua
After chromosome classification, a karyotype image can be produced. This is a grayscaled image associated to a  LUT, to be displayed with false colors:
Image5D can export a rgb image from the five channels image:
RGB image generated after background substraction of the five raw images