Generating examples of two overlapping chromosome from single chromosomes:
Notebook:
A notebook showing the different steps to generate overlapping examples is available on kaggle. As well as the raw images.
The idea:
Images of single chromosomes hybridized with with telomeric Cy3-probe and DAPI counterstained were extracted from the image of a metaphase:
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| DAPI stained chromesomes (left), (CCCTAA)3-PNA-Cy3 probes (Red) |
The monochrome DAPI and Cy3 images were added. Single chromosomes were isolated:
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| All images are 131x128 |
Given two single chromosomes, examples of overlapping chromosomes were generated combining rotations and translations:
Each greyscale image of a pair of overlapping chromosomes, is associated with a mask containing labels. The pixels of value equal to 3 maps to the overlapping domain of the two chromosomes:
Once downloaded and uncompressed, the examples can be loaded as a hdf5 file as shown bellow:
